Orally Active Oter Proteins Produced in Transgenic Plants

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Orally Active Oter Proteins Produced in Transgenic Plant -

Antigen encoding gene	Organism	Expressed in	Antigenic activity tested	Remarks
Integrated in Plant Genome
Surface antigen (HBsAg)	Hepatities B Virus	Tobacco (0.02% of total leaf protein)	Orally; S-IgA production in saliva stimulated	-
Surface antigen (HBsAg)	Hepatitis B virus	Tobacco (as 22 nm virus-like particles)	Parenterally in mice; similar to that of yeast derived HBsAg	Both B- and T-cell epitopes preserved
Heat-liable enterotoxin B subunit (LT-B)	E.coli	Tobacco,potato (low level of expression)	Orally in mice;comparable to bacteria-derived LT-B	A proportion of LT-B is pentamerised
Viral Vectors
a.Foreign gene transcription (driven by subgenomic promoter of TMV)
α-Trichosanthin	Chinese medicinal plant	High level of expresion	-	An antiviral protein
b. Capsaid protein fusions
Malarial epitope	Malarial sporozoties	Tobacco, (TMV capsaid protein fusions)	By ELISA,Western blot,positive	Both internal and C-terminal fusions
ZP3 protein epitope (13 amino acids)	Mammalian oocyte zona pellucida	Tobacco (TMV capsid protein fusion); high expression	Parenterally in mice; antibodies produced	Ovarian pathology; no effect on fertility
HIV-1 epitope	Human immunodeficiency	Fusion with small coat protein of cowpea mosaic virus	Immunogenic in animals	CPMV-HIV-1 antibodies active aginst 3 strains of HIV-1
Rhinovirus 14 epitope	Human rhinovirus	(CPMV)*