Transgenic Plants

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 Transgenic Plants - Part II

  Application of Transgenic Plants

  Gene Constructs

  Typical Plant Gene

  Promoters OR Enhancers

  Reporter Genes

 Promoters Used for Driving the Expression of Transgenes in Different Plant Species

  Examples of Regulatory Sequences Determining Tissue Specific and /or stimulus specific gene expression

  Important Genes / Sequences of pTi ( nopaline Type) and their Functions

  Integration of T-DNA into Plant Genome

  Vectors Derived from pTi

  Disarming

  Co-integrate pTi Vectors

  Features of Co-integrate and Binary Vectors of pTi

  Binary Vectors

  Plant Virus Vectors

  Cauliflower Mosaic Virus (CaMV)

  Tobacco Mosaic Virus (TMV)

  Brome Mosiac Virus (BMV)

  Integration of Transgenes

  Inheritance of Transgenes

  Analysis and Confirmation of Transgene Integration

  Genetic Transformation of Chloroplasts

 Vectors for Chloroplast Transformation

  Chloroplast Transformation Methods

  Advantages of Chloroplast Transformation

  Limitations of Chloroplast Trasnformation

  Examples of Useful Gene Transfers

  Herbicide Resistance

  Insect Resistance

  Mechanism of Action of Selected Herbicides and the basis of Generating Resistance against them in Transgenic Plants

  Target Molecules Resistant to the Herbicide

  Overproduction of the Target Molecule

  Detoxification OR Degradation of Herbicide

  Crystal (Cry) Proteins

  Toxic Action of Cry Proteins

  Expression of Cry Genes in Plants

  Cry Genes of B.thuringiensis their Proteins and Target Insects

  Insect Resistance to Cry Proteins

  Low Levels of Expression of Cry Genes

  Other Genes for Insect Resistance

  Virus Resistance

  Resistance to Bacterial and Fungal Diseases

  Comparison of the Various Strategies to Obtain Virus Resistant Transgenic Plants

  Drought Resistance

  Modification of Seed Protein Quality

  Golden Rice

  Suppression of Endogenous Genes

  Antisense Gene Approach

  Co suppression of Genes

  RNA Mediated Interference (RNAi)

  Biochemical Production

  Hirudin - A Polypeptide

  Phytase - An Enzyme

  Important Transgenes, Which Modify Starch/Lipid OR produce a Novel Non Protein Compound in Plants

  Some Important Trangenes Encoding Valuable Polypeptides /Enzymes Expressed in Plants

  Polyhydroxybutyrate, A Biodegradable Plastic Substrate

  Problems in Biochemical Production

  Advantages of Biochemical Production in Plants

  Plant Derived Vaccines

  Edible Vaccines

  Recombinant and Subunit Vaccines

  Status of Plant Derived Vaccines

  Problems in Gene Transfer

  Gene Silencing

  Orally Active and Other Transgenic Proteins Produced in Transgenic Plants

  Problems Due to Gene Silencing

  Amelioration of Gene Silencing

  Safety Regulation for Transgenic Plants

  Genetically Engineered Crop Varieties Approved for Commercial Cultivation and Allowed in the US Food Supply

  Total Area Under Transgenic Crop Varieties in Different Countries

Transgenic Plants

Transgenic Plants - Conventionally, the genetic variation necessary for crop improvement is generated through hybridization, mutagenesis and polyploidy. More recently, somatic hybridization and somaclonal variation have been used for this purpose.

However, the most potent biotechnological approach for creating the desired is the transfer of specifically constructed gene assemblies through various transformation techniques; this constitutes genetic engineering.

The plants obtained through genetic engineering contain a gene or genes usually from an unrelated organism; such genes are called transgenes, and the plants containing transgenes are known as transgenic plants.

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  List of Some of the Marker Genes Used for Gene Transfers into Plants

  Vectors for the Production of Transgenic Plants

  Problems Posed by Antibiotic Resistance Reporter Genes

  Plasmid Vectors

  Molecular Biology of Agrobacterium Infection

  Properties of Crowngall Cells

  Ti Plasmid

  Organisation of T-DNA

  Organisation of vir Region

  Transfer of TDNA

  Transformation Techniques

  Agrobacterium Mediated Gene Transfer

  Direct Gene Transfers

  Electroporation

  Particle Gun Method

  Lipofection

  Microinjection

  Fibre Mediated DNA Delivery

  DNA Delivery via Growing Pollen Tubes

  Macroinjection

  Direct DNA Uptake by Mature Zygotic Embryos

  Comparison of Some of the Important DNA Delivery Techniques for Plant Cells