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Transgenic Plants for Molecular Forming - An additional major goal. of biotechnology industry is also the use of transgenic plants as 'factories' for manufacturing speciality chemicals and pharmaceuticals. Sugars, fatty acids, starches, celluloses, rubber and wax are traditionally obtained from plants and genetic engineering can be used to increase their production.
Following are some of the examples:
(1) Transfer of a gene for mannitol dehydrogenase from E. coli to tobacco was achieved, which led to increase in the level of mannitol in transgenic tobacco plants.

(2) Transfer of bacterial gene (from Klebsiella) for cyclodextrin glucosyltransferase (CGTase) to potato was achieved successfully, leading to the production of α- and β cyclodextrins (CDs) in potato tubers.

Cyclodextrins are cyclic oligosaccharides containing six, seven or eight glucose molecules in α, β or γ CDs respectively. CDs can be obtained from starch by the action of cyclodextrin glucosyl transferase (CGTase) enzyme, and are useful for pharmaceutical delivery systems, flavour and odour enhancement and for removal of undesired compounds (such as caffeine) from foods.
The major use of CDs is due to their ability to form inclusion complexes with a wide variety of organic compounds. Tissue specific expression of CGTase in potato tuber and its targeting to plastids for action on stored starch was achieved through, the use of a chimeric gene construct consisting of the following:
(i) a patatin promoter (for tuber specific expression of CGTase gene);
(ii) a DNA sequence encoding small subunit of ribulose bisphosphate carboxylase (SSU) transit peptide,

(iii) the CGTase structural gene from Klebsiella and
(iv) nopaline synthase (nos) 3' region.
More transgenic plants with CGTase gene may be produced in view of the present high cost and world demand of CDs and due to availability of a number of CGTase genes.

(3) Transfer of genes for acetoacetyl CoA reductase (phb B) and polyhydroxybutyrate (PHB) synthase (phb C), which catalyze two steps in the production of polyhydroxybutyrate or PHB (a biodegradable thermoplastic polymer).These genes were successfully transferred and their expression was demonstrated in transgenic plants of Arabidopsis thaliana.

(4) Chimeric genes having CaMV promoter and encoding human serum albumin (HSA) were transferred successfully and transgenic potato and tobacco plants obtained. The secretion of protein was achieved by using either the human preprosequence or the signal sequence from extracellular PR -S protein from tobacco. HSA was secreted in transgenic leaf tissue.

(5) The production of pharmaceutically active compounds like enkephalins was achieved in transgenic oil seed rape.