Transgenic Plants in Monocotyledons - Production of transgenic plants in monocotyledons was initially not possible due to the following two limitations:
(i) monocotyledons are ordinarily not infected by Agrobacterium, which was so widely used in dicotyledonous plants for carrying Ti plasmids for transformation, and

(ii) the regeneration of plants from protoplasts or single cells, which are commonly used for transformation, was not possible.

Both these limitations have been overcome, since alternative methods for DNA uptake have now been developed and regeneration protocols for crops like rice and maize have been successful during the last few years.