Acetone OR Butanol Production,Genus Clostridium,Starch, Molasses, Sucrose, Wood Hydrolysates,Alcohol,Ethanol,

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Use Of Microbes in Industry and Agriculture Part 1
Use Of Microbes in Industry and Agriculture
Microorganism Isolation Methods
Microorganism Sterilization and Disinfection Methods
Microorganism Culture Methods
Acetone OR Butanol Production
Gluconic Acid Production
Production of Other Organic Compounds
List of Organic Compounds Produced by Microbial Fermentation
Production of Alpha Amylases
Production of Alkaline Proteases
Production of Neutral Proteases
Production of Acid Proteases
Production of Lipases
Bacteria and Fungi Producing Alpha Amylase

	Acetone OR Butanol Production - Acetone, butanol, butyric acid and isopropanol are obtained through fermentation of starch, molasses, sucrose, wood hydrolysates and pentoses using bacteria belonging to the genus Clostridium. The relative proportion of different products varies with the species and the strain of Clostridium used. The biosynthetic steps involved in the production of each of these compounds are interrelated, all of them being derived from acetyl CoA produced from either glucose or fatty acid degradation.
In a typical fermentation process, the substrate includes molasses plus maize steep liquor, which are taken in a suitable container which is gassed with C02..The fermentation is carried out at 33-35°C, with initial pH of 5.8 to 6.0. However, the pH drops in the first 18 hours to about 5.2 due to the production of acetic and butyric acids, but in the next 18 hours increases again due to the production of acetone and butanol from these acids. Contamination due to bacteriophages and lactobacilli is a common problem in such fermentation and therefore absolute sterilization is necessary.
In India, alcohol produced from molasses is used as a feedstock for production of acetone and butanol. 1.5-1.9 MT of alcohol is used per MT of butanol and 2.2 MT of alcohol is used per MT of acetone. Acetone, butanol and ethanol are recovered through continuous distillation and fractionation. Carbon dioxide produced is recovered and converted into liquid CO₂ or dry ice. The residue left after the distillation may be dried and utilized as animal feed.