Azolla with Anabaena has been used with some success, but there are following limitations:
(i) Azolla, as a green manuring crop is labour intensive;
(ii) raising of Azolla needs assured and adequate supply of water;
(iii) damage of Azolla (in the nursery) is caused by pest diseases, heat, etc.
(iv) optimum temperature is required for Azolla multiplication.
Phosphate solubilising bacteria (PSB, e.g. Thiobacillus, Bacillus spp., etc.) and plant growth promoting rhizobacteria (PGPR), including Pseudomonas fluorescens and P. putida are important new biofertilizers. PSBs convert non-available inorganic phosphates into soluble organic phosphates, which can be utilized by crop plants.
PGPRs produce siderophores (iron chelating substances, e.g. pseudobactin), which chelate with iron and' make it unavailable to harmful fungi (e.g. Eriwinia) in rhizosphere, leading to their death; PGPRs have resulted in an increase in yields of potato (30%), radish (60-144%) and sugarbeet (4-8t/ha). These biofertilizers are yet to be commercially utilized in our country.



