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Methane Production Using Microbial Systems - Methane is produced during anaerobic decomposition of sewage and other organic wastes by bacteria. The methane thus produced is collected and used as fuel in many countries.

The methogenic bacteria are able to utilize acetate, methanol, formate, and Hz + CO2 of the organic wastes for the production of methane gas. The starting materials in the wastes are, however, complex organic molecules such as cellulose, starch, fats and proteins. These are first broken down to simpler substrates such as acetate and H2 + CO2 by other microorganisms.

In the sewage treatment system, the degradation of macromolecules (cellulose, protein, fats, etc.) to smaller molecules is called liquefaction phase. A large number of microorganisms can perform this degradation. However, only a restricted group of organisms are able to produce methane from the degraded products.

The composition and the amount of the gas (called biogas), produced from the degradation of biological products, is related to the quality of the substrate and the temperature. In anaerobic sludge digestion process, upto 600 litres of methane is produced per kg dry organic matter.

The biogas generated in such a process contains 60-70% methane, 25-30% CO2 and the rest is H2 + N2' In our own country, small and inexpensive installations have been set up in the villages to convert cow dung into biogas. About 200 to 1000 litres of methane per kg dry cow dung is produced at 55°C, in such installations.

In most systems, the amount of biogas generated increases with the increase in temperature. The time for the generation of gas is also reduced at high temperatures.