Sterilization is necessary because contamination can cause the production of undesired substances or can suppress the desired reaction. The end products are generally secreted outside the cell and' they remain either dissolved or suspended in the fermentation broth. For further processing, if fungus is the micro organism used, it is separated by filtration.
Recovery of the product is achieved by precipitation as calcium salt, by adsorption to ion exchangers, by extraction with appropriate solvents or by direct distillation from the medium (for volatile substances).
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