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Microbial Transformations - Micro organisms have the ability to convert one organic molecule into another. Many complex bioconversions that are achieved by micro organisms, can not be achieved by normal chemical means and are achieved with great difficulty by using organisms other than microbes. Microbial trans­formations of the molecules which have found industrial applications involve oxidation, reduction, isomerization, hydrolysis, condensation, etc.

Transformation of organic compounds are achieved by using the spores, growing cultures, resting cells, enzymes, immobilized cells or enzymes derived from micro organisms. Transformation reactions at large scale are carried out under sterile conditions in aerated and stirred fermenters.

Sterilization is necessary because contamination can cause the production of undesired substances or can suppress the desired reaction. The end products are generally secreted outside the cell and' they remain either dissolved or suspended in the fermentation broth. For further processing, if fungus is the micro organism used, it is separated by filtration.

Recovery of the product is achieved by precipitation as calcium salt, by adsorption to ion exchangers, by extraction with appropriate solvents or by direct distillation from the medium (for volatile substances).