Microorganism Isolation Methods,Soil Dilution,Gradient Plate Method,Aerosol Dilution,Fungi,Bacteria,Sporulating Bacteria

www.molecular-plant-biotechnology.info

Back to Home

Home >>Use Of Microbes in Industry and Agriculture >> Microorganism Isolation Methods

Back to Home

Use Of Microbes in Industry and Agriculture Part 1
Use Of Microbes in Industry and Agriculture
Microorganism Isolation Methods
Microorganism Sterilization and Disinfection Methods
Microorganism Culture Methods
Acetone OR Butanol Production
Gluconic Acid Production
Production of Other Organic Compounds
List of Organic Compounds Produced by Microbial Fermentation
Production of Alpha Amylases
Production of Alkaline Proteases
Production of Neutral Proteases
Production of Acid Proteases
Production of Lipases
Bacteria and Fungi Producing Alpha Amylase

	Microorganism Isolation Methods - Micro organisms are though ubiquitous in their occurrence, common sources for their isolation are soils, lakes and river muds. Common techniques used for the isolation of industrially useful micro organisms include the following: (i) direct sponge of the soil; (ii) soil dilution; Micro organisms are though ubiquitous in their occurrence, common sources for their isolation are soils, lakes and river muds. Common techniques used for the isolation of industrially useful micro organisms include the following: (i) direct sponge of the soil;
(ii) soil dilution; (iii) gradient plate method (pour plate and streak plate techniques); (iv)aerosol dilution; (v) flotation; (vi) centrifugation. Although for the detailed description of the above techniques, the readers are advised to consult a book on practical microbiology, they should recognize that the technique of isolating micro-organisms also varies according to the nature and physiological properties of the microbe to be isolated.
For example, isolation of fungi from a mixture of fungi and bacteria can be achieved easily by incorporating in the growth medium, antibiotics to which the bacteria are sensitive. Similarly, from a mixture of sporulating (spore forming) and non-sporulating bacteria, spore forming bacteria can be isolated by heating the mixture to 70 - 80° C for 5-10 min, leading to the death of all vegetative forms, so that the remaining spores will ,belong to only sporulating bacteria. Small samples of microbial cultures can also be obtained from permanent cultures, which are maintained by government as well as private agencies in most countries. (iii) gradient plate method (pour plate and streak plate techniques); (iv)aerosol dilution; (v) flotation; (vi) centrifugation.
Although for the detailed description of the above techniques, the readers are advised to consult a book on practical microbiology, they should recognize that the technique of isolating micro-organisms also varies according to the nature and physiological properties of the microbe to be isolated. For example, isolation of fungi from a mixture of fungi and bacteria can be achieved easily by incorporating in the growth medium, antibiotics to which the bacteria are sensitive. Similarly, from a mixture of sporulating (spore forming) and non sporulating bacteria, spore forming bacteria can be isolated by heating the mixture to 70 - 80° C for 5-10 min, leading to the death of all vegetative forms, so that the remaining spores will ,belong to only sporulating bacteria. Small samples of microbial cultures can also be obtained from permanent cultures, which are maintained by government as well as private agencies in most countries.