Procedure OR Steps for Commercial Production of Antibiotics - A small sample of the cultured stock is taken and the amount of material is amplified by growth on solid surfaces or in liquid culture. Spores of the micro organism are collected and retained in a suspension form. This suspension is transferred into a container suitable for attachment to the fermenter.

Different media are required at different stages of antibiotic production.Manufactures spend considerable amount of time and money in developing media both for increasing antibiotic productivity and for decreasing costs.