Production of Alkaline Proteases - They are produced and secreted out by many bacteria and fungi. The most important producers are species of Bacillus. (e.g. B. licheniformis, B. amyloliquefaciens, B. firm us, B. megaterium, and B pumilus), species of Streptomyces (e.g. S. fradiae, S. griseus, and S. rectus) and some fungi (e.g. Aspergillus niger, A. sojae, A. oryzae and A flavus).

The cultures are maintained at 3O-37°C in fermenters or flasks, where production of a protease is chiefly regulated by the composition of culture medium. High partial pressure of oxygen is generally necessary for optimal protease production. In most cases, the enzymes are collected, purified and immobilized before their use in the detergent industry.