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Transformations of Steroids and Sterols - Most steroids have hormone properties and include glucocorticoids, mineralocorticoids, androgens, estrogens, progesterones, etc. All these steroids have a basic molecular skeleton, which is cyclopentano per hydrophenanthrene or sterane nucleus, One of the steroid hormone is progesterone, which is quite active during pregnancy. On industrial scale it acts as a precursor for producing at least four other products, using micro organisms.

These conversions involve hydroxylation and dehydrogenation. The hydroxylated dehydrogenated products have better efficacy as hormone.

For example, the oxygen at C-l1 is essential for the anti-inflammatory effect of cortisone (another steroid hormone). The cortisone itself can be produced from progesterone and then modified. In 1949, 1.0g of cortisone was produced at a cost of about 200 US dollars, but as a result of introduction of the microbial process for the C-ll -hydroxylation of progesterone, the cost had decreased to under 1 US dollar by 1979.

Currently, almost all positions of the steroid molecules can be specifically hydroxylated by different micro organisms.

Steroid hormones can also be produced from sterol precursors which are quite inexpensive, For instance, diosgenin, β -sitosterol, cholesterol, etc are often used as hormone precursors, These sterols have the same sterane nucleus and one or more side chains.

The basic approach in their microbial bio conversion is to remove the side chain. Production of some steroid hormones using cholesterol as primary molecule with the help of mycobacteria is diagrammatically represented.