Steroid hormones can also be produced from sterol precursors which are quite inexpensive, For instance, diosgenin, β -sitosterol, cholesterol, etc are often used as hormone precursors, These sterols have the same sterane nucleus and one or more side chains.
The basic approach in their microbial bio conversion is to remove the side chain. Production of some steroid hormones using cholesterol as primary molecule with the help of mycobacteria is diagrammatically represented.



